1. What is the smallest amount of DNA I can put into TruePrime® reaction? Typically, the minimum amount of DNA for successful WGA or RCA with TruePrime® is 1 pg. However, amplification is dependent upon the quality of input DNA. Optimal amplification is achieved with 1 ng or more of DNA template. 2. Which is the…

1. Is SunScript® RT compatible with other DNA polymerase to use it in a single-tube reaction? There is no problem to use our SunScript® enzyme together with other DNA Polymerase, it should work. In fact, we have a One-Step RT-PCR kit in which we combine SunScript with a High Fidelity Taq Polymerase to perform both…

1. Is QualiPhi ® DNA Polymerase supplied with dNTPs? No. The kit only contains the enzyme and reaction buffer. 2. Is QualiPhi ® DNA Polymerase supplied with random primers? No. The kit only contains the enzyme and reaction buffer. 3. What type of DNA template can be amplified by QualiPhi ® DNA Polymerase? Both linear…

1. Is MagniPhi® DNA Polymerase supplied with dNTPs? No. The kit only contains the enzyme and reaction buffer. 2. Is MagniPhi® DNA Polymerase supplied with random primers? No. The kit only contains the enzyme and reaction buffer. 3. What type of DNA template can be amplified by MagniPhi® DNA Polymerase? Both linear and circular DNA…

Can I use the amplified DNA obtained with CovCheck to prepare NGS libraries? The amplification products obtained with the CovCheck™ are not suitable to prepare libraries. The idea is to spend part of your amplified DNA to check the quality with CovCheck™ and then use the rest to prepare the library for any sequencing platform….